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In Stock Series | Precisely Target E. coli: A Versatile Chromogenic System with Multiple β-D-Glucuronidase (GUS) Substrate Options
2026/1/23 16:59:20 Browse volume(38)β‑D‑Glucuronidase (β‑GUS)
In microbial detection, β-D-glucuronidase (β-Glucuronidase, β-GUS) is an enzyme with high indicative significance. It is highly associated with Escherichia coli (E. coli) and is widely used in food, drinking water, and clinical microbial detection systems, serving as a typical indicator enzyme and identification marker enzyme.
By detecting the presence and activity of β-GUS, one can quickly determine the existence of target microorganisms and convert their metabolic characteristics into recognizable detection signals, thus it is also regarded as a "microbial metabolic fingerprint".
β-D-glucuronidase is a hydrolase that specifically cleaves β-D-glucuronidic bonds. The distribution of this enzyme in nature has clear microbial taxonomic significance:
Primary Source: The vast majority (>97%) of Escherichia coli (E. coli) constitutively express this enzyme.
Diagnostic Value: Within the Enterobacteriaceae family, many important pathogens such as most strains of Salmonella and Shigella do not produce this enzyme. This difference in activity makes it a specific enzymatic marker for distinguishing E. coli from other coliforms and some enteric pathogens.
Modern microbial detection systems typically require: speed, accuracy, and visualizability.
β-GUS possesses all these core features:
Rapid Reaction: The enzymatic reaction is fast, allowing for color or fluorescence release in a short time.
High Specificity: Highly correlated with E. coli, low false-positive rate, suitable for rapid screening of food and water quality.
Easy Interpretation: Results are directly observable via color or fluorescence signals or read by instruments.
For these reasons, β-GUS has become one of the most classic and stable indicator enzymes in chromogenic media and rapid detection methods.
The core of detecting this enzyme's activity lies in using artificially synthesized enzyme substrates. These substrates share a unified molecular structure consisting of two parts:
Enzyme Cleavage Recognition Site: The β-D-glucuronic acid group ensures the substrate is specifically hydrolyzed only by the target enzyme.
Signal Reporter Group: A chromophore or fluorophore attached via the glycosidic bond.
Reaction Principle:
Under the action of β-D-glucuronidase, the glycosidic bond of the substrate molecule is cleaved, releasing the reporter group. This reporter group:
Chromogenic Type (e.g., indole derivatives): Can form insoluble colored precipitates (e.g., indigo blue) through oxidative coupling, enabling in-situ coloration on plates or in media.
Fluorogenic Type (e.g., coumarin derivatives): Emits strong fluorescence under appropriate excitation wavelengths, suitable for high-sensitivity instrument quantitative analysis.
1. X-GlcA Sodium Salt Anhydrous
Chinese Name: 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid, sodium salt (anhydrous)
English Name: 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid, sodium salt (anhydrous)
Abbreviation: X-GlcA Sodium Salt Anhydrous
CAS Number: 129541-41-9
Molecular Formula: C14H12BrClNNaO7
Molecular Weight: 444.59
Specifications: 1 g / 25 g / 100 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01010006
Stock Status: In Stock
Color: Dark Blue
Application: Classic β-D-glucuronidase chromogenic substrate, widely used for E. coli detection in food and water.
2. X-GlcA Sodium Salt Trihydrate
Chinese Name: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt trihydrate
English Name: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt trihydrate
Abbreviation: X-GlcA Sodium Salt Trihydrate
CAS Number: 370100-64-4
Molecular Formula: C₁₄H₁₂BrClNNaO₇ · (H₂O)₃
Molecular Weight: 498.64
Specifications: 1 g / 5 g / 25 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01010005
Stock Status: In Stock
Color: Dark Blue
Application: Used for detecting β-D-glucuronidase-producing microorganisms. The trihydrate form offers better stability, suitable for chromogenic media and microbial solid plate detection systems.
3. Magenta-GlcA Sodium Salt
Chinese Name: 5-Bromo-6-chloro-3-indolyl β-D-glucuronide sodium salt
English Name: 5-Bromo-6-chloro-3-indolyl β-D-glucuronide sodium salt
Abbreviation: Magenta-GlcA Sodium Salt
CAS Number: 216971-54-9
Molecular Formula: C14H12BrClNNaO7
Molecular Weight: 444.59
Specifications: 1 g / 5 g / 25 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01020013
Stock Status: In Stock
Color: Magenta
Application: Special color spectrum β-D-glucuronidase chromogenic substrate. Primarily used for the development of dual or multiplex chromogenic media.
4. PNP-GlcA
Chinese Name: 4-Nitrophenyl β-D-glucuronide
English Name: 4-Nitrophenyl β-D-glucuronide
Abbreviation: PNP-GlcA
CAS Number: 10344-94-2
Molecular Formula: C12H13NO9
Molecular Weight: 315.23
Specifications: 1 g / 5 g / 25 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01060026
Stock Status: In Stock
Color: Yellow
Application: Suitable for liquid colorimetric quantitative detection of β-D-glucuronidase activity, commonly used in enzyme kinetics studies or quantitative assay kit development.
5. Y-GlcA CHX Salt
Chinese Name: 3-Indolyl β-D-glucuronide cyclohexylammonium salt
English Name: 3-Indolyl β-D-glucuronide cyclohexylammonium salt
Abbreviation: Y-GlcA CHX Salt
CAS Number: 216971-58-3
Molecular Formula: C20H28N2O7
Molecular Weight: 408.45
Specifications: 1 g / 5 g / 25 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01040014
Stock Status: In Stock
Color: Indigo Blue
Application: Basic indole-type substrate, with salt form design optimized for solubility and stability, suitable for chromogenic media formulation development.
6. Salmon-GlcA Sodium Salt
Chinese Name: 6-Chloro-3-indolyl β-D-glucuronide sodium salt
English Name: 6-Chloro-3-indolyl β-D-glucuronide sodium salt
Abbreviation: Salmon-GlcA Sodium Salt
CAS Number: 216971-56-1
Molecular Formula: C14H13ClNNaO7
Molecular Weight: 365.70
Specifications: 1 g / 5 g / 25 g (Custom packaging supported)
Form: Lyophilized Powder
Cat. No.: G01030007
Stock Status: In Stock
Color: Salmon Red
Application: Suitable for multi-color chromogenic culture systems, can form multi-target detection schemes with blue, magenta substrates.
Extended Services
Chromogenic for fast qualification, fluorogenic for accurate quantification — β-GUS substrates, the gold standard solution for microbial detection.
For more chromogenic substrates or customized packaging services, please call +86 13302967066!
GeneSeqTools Bioscience & Technology is dedicated to providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.
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