Chinese Name: 2-Nitrophenyl α-D-galactopyranoside

English Name: 2-Nitrophenyl α-D-galactopyranoside

Abbreviation: ONP-α-Gal

CAS Number: 19887-85-5

Molecular Formula: C12H15NO8

Molecular Weight: 301.25

Specifications: 10 g / 25 g / 100 g (Custom packaging supported)

Form: Lyophilized Powder

Cat. No.: G01060019

Color: Yellow

Stock Status: In Stock

ONP-α-Gal, as a classic chromogenic substrate for α-galactosidase, integrates clear reaction mechanism, mature detection methods, and highly reproducible results. It is an important basic reagent for establishing quantitative models of enzyme activity, evaluating enzymatic parameters, and conducting microbial functional research.

The molecular structure of ONP-α-Gal contains a stable α-glycosidic bond, which can be specifically hydrolyzed by α-galactosidase. When the substrate is cleaved by the enzyme, o-Nitrophenol is released, exhibiting a distinct yellow absorption peak under alkaline conditions, allowing for precise detection via spectrophotometry. This "substrate cleavage-color development amplification" reaction mode makes ONP-α-Gal an ideal choice for constructing colorimetric enzyme activity detection systems, particularly suitable for quantitative analysis and enzyme kinetics studies.

Compared with fluorogenic substrates, ONP-α-Gal offers advantages such as lower detection equipment requirements, high data stability, and controllable cost, holding an irreplaceable position in basic research, teaching experiments, and standardized method validation. Whether used for α-galactosidase screening, mutant activity comparison, or microbial enzyme phenotype research, ONP-α-Gal is a classic, cost-effective, and reliable tool substrate.

· Quantitative detection of α-galactosidase activity

· Determination of enzyme kinetic parameters (Km, Vmax)

· Functional screening of microbial strains

Enzyme kinetics research is highly sensitive to substrate purity and batch consistency; minor differences can cause systematic deviations in Km values and reaction rates. The ONP-α-Gal provided by GeneSeqTools Bioscience & Technology is prepared using standardized processes, with stable product characteristics and high inter-batch consistency, suitable for building long-term stable detection systems.

We offer multi-specification packaging of 10 g / 25 g / 100 g and support customized packaging services, helping scientific research and industrial users flexibly match experimental scales, reducing reagent waste and project interruption risks. The in-stock supply system ensures project continuity and provides reliable support for experimental progress.