Chinese Name: Kallikrein (KLK) Substrate PFR-PNA

English Name: H-D-Pro-Phe-Arg-pNA · 2HCl

Abbreviation: S-2302

CAS Number: 62354-56-7

Molecular Formula: C26H36Cl2N8O5

Molecular Weight: 611.53

Specification: 1 mg / 5 mg / 100 mg (custom packaging supported)

Form: Lyophilized powder

Product Number: G04010301

Color Development: Yellow

Stock: In stock

Chromogenic substrate S-2302 (H-D-Pro-Phe-Arg-pNA · 2HCl, 62354-56-7) is a classic kallikrein colorimetric substrate. It enables rapid and quantitative detection of KLK activity through colorimetry and is one of the most mature and reliable substrates used in kinin system research.

This product is supplied in lyophilized powder form, with high stability, easy storage, and easy reconstitution. It is suitable for various buffer systems and detection systems. Whether for routine enzyme activity assays, kinetic parameter analysis, or inhibitor screening experiments, S-2302 provides good signal-to-noise ratio and inter-batch consistency, offering a solid data foundation for experimental results.

Mechanism: Kallikrein recognizes and hydrolyzes the amide bond between the C-terminal arginine (Arg) and p-nitroaniline (pNA) of the substrate, releasing yellow pNA.

Detection: As the reaction proceeds, the solution turns yellow. By monitoring the absorbance change at OD 405 nm, enzyme activity can be quantified. It has high sensitivity and a good linear range.

✅ In Stock: Multiple specifications are regularly stocked, significantly shortening procurement and experimental preparation cycles.

✅ Classically Validated: S-2302 is one of the standard substrates long used in kallikrein research, and data can be directly benchmarked against international mainstream databases.

✅ High Specificity: Has good selectivity for plasma kallikrein.

✅ High Purity: Full-process HPLC quality control, HPLC purity ≥98%, ensuring inter-batch consistency.

✅ Easy Operation: No fluorescence or luminescence instruments required; quantitative analysis can be performed with a common microplate reader.

· Quantitative detection of kallikrein (KLK) activity

· Functional study of the plasma kinin system

· KLK inhibitor screening and pharmacodynamic evaluation

· Research on contact activation pathway/endogenous coagulation pathway