Chinese Name: Disodium 4-Nitrophenyl Phosphate

English Name: Disodium 4-Nitrophenyl Phosphate

Abbreviation: pNPP

CAS Number: 4264-83-9

Molecular Formula: C6H4NNa2O6P

Molecular Weight: 263.05

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Product Number: G03010026

Stock: In stock

Color: Yellow

pNPP is a typical phosphoric ester enzyme substrate. Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of its phosphoester bond, producing p-Nitrophenol and inorganic phosphate.

The produced p-Nitrophenol appears yellow in an alkaline environment and has a distinct absorption peak at 405 nm. By measuring the change in absorbance with a microplate reader or spectrophotometer, alkaline phosphatase activity can be quantitatively analyzed. This reaction system is stable and sensitive, and has become a standard chromogenic system in various immunoassays.

• Enzyme Activity Studies
For quantitative determination of alkaline phosphatase activity and enzyme kinetic studies.

• IVD Reagent Development
Used in the development of in vitro diagnostic kits based on alkaline phosphatase labeling systems.

• ELISA Detection
As a common chromogenic substrate for alkaline phosphatase labeling systems, used for signal detection in enzyme-linked immunosorbent assays.

The biggest pain point when using pNPP substrate is: if the raw material purity is insufficient or contains free impurities, the prepared solution will have a "yellow background", severely compressing the effective reading range of the experiment. GeneSeqTools Bioscience & Technology provides customers with stable product supply and quality assurance:

• Low Background, High Signal: Strictly optimized synthesis and purification processes effectively control residual impurities such as free p-nitrophenol. The product has extremely low background absorption, ensuring purer detection signals, improving the signal-to-noise ratio, and reducing background interference.

• In Stock: Common specifications (1 g / 5 g / 25 g) are kept in ample stock, shipped immediately upon order, avoiding delays in R&D progress due to supply cycles.

• Flexible Customization: Custom packaging and large-scale supply can be provided according to the needs of research institutions or IVD companies, supporting the transition of projects from the R&D phase to industrialization.