Chinese Name: Bis(2,2'-bipyridine-ΚN1,ΚN1')(dipyrido[3,2-a:2',3'-c]phenazine-ΚN4,ΚN5)ruthenium(2+)

English Name: Bis(2,2'-bipyridine-N1,N1')(dipyrido[3,2-a:2',3'-c]phenazine-N4,N5)-Ruthenium(2+), bis(hexafluorophosphate) complex

Abbreviation: Ru(bpy)2dppz(PF6)2

CAS Number: 87564-74-7

Molecular Formula: C38H26P2F12N8Ru

Molecular Weight: 985.10

Specifications: 10 mg / 100 mg / 1 g (Custom packaging supported)

Form: Powder

Product Number: G06030011

Stock: In stock

Luminescence: Orange-red

Ru(bpy)2dppz(PF6)2 is a classic ruthenium(II) polypyridyl complex luminescent probe. Due to the dppz ligand forming hydrogen bonds with water molecules in aqueous solution, its luminescence is almost completely quenched. However, when it intercalates into the base stack of double-stranded DNA, the microenvironment around the ligand changes, inhibiting non-radiative quenching processes and resulting in a significantly enhanced orange-red luminescence signal.

Due to this property, this molecule serves as a classic molecular probe for DNA binding studies and nucleic acid detection, finding wide application in biochemical and molecular biology research.

• Nucleic Acid Quantification and Structural Analysis
  As a classic DNA "light-switch" probe, used for DNA presence determination, quantitative detection, DNA structural changes, and DNA damage analysis.

• Photophysical and Photochemical Studies
  As a typical Ru(II) polypyridyl complex, it serves as a textbook-level reference model for studying metal-to-ligand charge transfer (MLCT), excited state lifetimes, solvent effects, and hydrogen bonding quenching mechanisms.

• Electrochemical Biosensor Development
  This complex exhibits excellent electrochemical activity, suitable for modifying electrodes. By detecting electrochemical signal changes upon DNA binding, it enables highly sensitive DNA mutation recognition and nucleic acid detection.

• Biomolecular Interaction Studies
  Used to study the intercalation binding modes and conformational changes of small molecules/proteins with DNA.

✅ Light-Switch Effect
Leveraging the unique mechanism of "quenching by hydrogen bonds in water, luminescence recovery in the hydrophobic DNA environment," the background luminescence is extremely low in the absence of nucleic acids. Upon intercalation into the DNA double helix, it rapidly releases orange-red fluorescence, enabling high signal-to-noise ratio detection.