Chinese Name: L-Pyroglutamic acid 7-amido-4-methylcoumarin

English Name: L-Pyroglutamic acid 7-amido-4-methylcoumarin

Abbreviation: Pyr-AMC

CAS Number: 66642-36-2

Molecular Formula: C15H14N2O4

Molecular Weight: 286.29

Specifications: 100 mg / 500 mg / 1 g (Custom packaging supported)

Form: Off-white crystalline powder

Product Number: G04050018

Stock: In stock

Signal: Blue fluorescence

Silent substrate → specific cleavage → fluorescence release

Pyr-AMC consists of L-pyroglutamic acid (Pyroglutamyl) coupled with AMC (7-amido-4-methylcoumarin) via an amide bond. Its core features are:

1. Enzyme recognition site: The pyroglutamyl terminus serves as the specific recognition sequence for pyroglutamyl aminopeptidase.

2. Fluorescent reporter group: AMC has extremely low background fluorescence before cleavage, and its fluorescence intensity increases significantly after cleavage.

3. Signal release mechanism:

• Pyroglutamyl aminopeptidase binds to Pyr-AMC, recognizing and hydrolyzing the amide bond between pyroglutamyl and AMC.

• Free AMC can be excited by an excitation light source (Ex≈350~365 nm), emitting a fluorescence signal (Em≈440~460 nm).

• Fluorescence intensity is linearly related to enzyme activity, allowing quantitative measurement of pyroglutamyl aminopeptidase activity.

This detection system does not require additional coupled enzymes; enzyme activity can be monitored directly through changes in fluorescence signal.

To meet the needs of pyroglutamyl aminopeptidase detection, GeneSeqTools Bioscience & Technology also provides a variety of pyroglutamyl aminopeptidase substrates:

✅ High purity control, ensuring detection consistency

• High purity quality control: Strict purification process with low impurity residue ensures product purity and batch consistency.

• Controllable background: Reduces the impact of non-specific fluorescence signals on detection results.

• Reliable results: Helps to improve experimental repeatability and data stability.

✅ Sensitive fluorescence response, clear detection signal

• AMC reporter group: Uses the classic AMC fluorophore with good fluorescence response characteristics.

• Clear signal: Direct release of fluorescence signal after cleavage facilitates monitoring of enzyme activity changes.

• Sensitive detection: Suitable for low enzyme activity and trace sample analysis.

✅ Specific recognition, adapted to pyroglutamyl aminopeptidase detection

• Precise recognition: Uses the pyroglutamyl group as the enzyme recognition site.

• Clear reaction: Specifically hydrolyzed by pyroglutamyl aminopeptidase.

• Convenient quantification: Changes in fluorescence signal are related to enzyme activity levels, facilitating the establishment of standardized detection systems.

✅ Mature application, compatible with multiple experimental platforms

• Mature and reliable method: The pyroglutamyl aminopeptidase test is a classic method for enterococcal identification, widely adopted in multiple microbiological standard operating procedures and literature.

• Rich literature foundation: The method for PGP-1 activity detection is mature, with over a hundred citations in PubMed using "Pyr-AMC" as a substrate.

• Good platform compatibility: Suitable for fluorescence microplate readers and microplate detection systems.

✅ High quality, competitive price, in-stock supply

• In-stock supply: Sufficient inventory on hand, shortening project procurement cycles and ensuring R&D and production progress.

• Price advantage: Leveraging the cost advantages of the domestic supply chain, the procurement cost for the same specifications is significantly lower than imported products.

• Customization support: Custom packaging services can be provided according to project requirements.