Chinese name: 5-Bromo-6-chloro-3-indolyl β-D-glucopyranoside

English name: 5-Bromo-6-chloro-3-indolyl β-D-glucopyranoside

Abbreviation: Magenta-Glc

CAS No.: 93863-89-9

Molecular Formula: C14H15BrClNO6

Molecular Weight: 408.63

Specification: 1 g/5 g/25 g (custom packaging supported)

Appearance: off-white crystalline powder

Catalog No.: G01020001

Signal: Magenta

Stock: In stock

Magenta-Glc is a β-D-glucosidase chromogenic substrate that can form a magenta precipitate through an enzymatic reaction, providing a color signal solution for the formulation development of microbial chromogenic culture media.

The role of Magenta-Glc can be understood from two stages: enzymatic hydrolysis and chromogenic reaction.

First, when β-D-glucosidase in the culture system can hydrolyze this substrate, the substrate releases the corresponding chromogenic group.

Subsequently, under oxidizing conditions, the released indoxyl intermediate further undergoes oxidative dimerization to form an insoluble magenta precipitate, causing the relevant colonies to present an observable color signal.

Therefore, the technical characteristic of Magenta-Glc is not only that it corresponds to a certain enzyme activity, but also that it can convert the corresponding enzymatic reaction into an observable magenta signal.

However, it should be noted that chromogenic substrates are not independent microbial identification reagents. The actual colony color is also affected by factors such as the microorganism's own enzymatic characteristics, culture medium formulation, and culture conditions.

In the above-mentioned Vibrio chromogenic culture medium patent, the β-D-glucosidase substrate and the β-galactosidase substrate undertake different reaction roles, and culture medium components such as sucrose also participate in the formation of colony phenotypes.

This provides an important idea for culture medium R&D: substrate selection cannot only look at which enzyme it corresponds to; it is also necessary to consider whether it can form the expected chromogenic result in the complete culture system.

It is necessary to consider whether it can form the expected chromogenic result in the complete culture system.

For Magenta-Glc, R&D personnel can focus on the following aspects:

Reaction differences between target and non-target bacteria

Even if both microorganisms may have β-D-glucosidase activity, their chromogenic performance in a specific culture medium may still be affected by formulation conditions. Therefore, the suitability of the substrate needs to be evaluated in combination with the actual reactions of target and non-target bacteria.

Combination of different chromogenic channels

When the culture medium needs to simultaneously utilize multiple enzymatic indicators, Magenta-Glc can be used as one option for the β-D-glucosidase chromogenic substrate and participate in formulation design together with other reaction channels.

Color signal and overall interpretation effect

Magenta is the chromogenic characteristic of Magenta-Glc, but the color itself does not directly equal microbial identification capability. Whether a valuable colony difference can be formed still requires comprehensive examination of background color development, colony phenotype, other formulation components, and culture conditions.

1. R&D of Chromogenic Culture Media for Vibrio parahaemolyticus and Other Pathogenic Vibrio

Magenta-Glc can be used as a β-D-glucosidase chromogenic substrate for formulation design and evaluation of chromogenic culture media for Vibrio parahaemolyticus and other pathogenic Vibrio.

2. R&D of Chromogenic Culture Media and Chromogenic Plates for Listeria

Magenta-Glc can be used in Listeria chromogenic culture systems and combined with other chromogenic substrates to form colony color signals, providing a raw material option for the development of Listeria chromogenic plates and related culture media for food processing environment monitoring.

3. Chromogenic Differentiation Research of Salmonella and Other Enterobacteriaceae

Magenta-Glc can be used for R&D of related microbial chromogenic culture media and substrate screening; in combination with β-D-glucosidase-related chromogenic reactions, it assists in evaluating the reaction differences between Salmonella and other Enterobacteriaceae.

✅ Clear application path, formulation development has references

Magenta-Glc not only has a clear β-D-glucosidase chromogenic reaction, but also has specific culture medium application cases. From literature on multi-enzyme chromogenic formulations for Vibrio chromogenic culture media to the application of combined substrates in commercial Listeria chromogenic plates, it provides specific references for substrate selection and formulation design of related culture media.

✅ Magenta chromogenic characteristic, expanding substrate combination options

Magenta-Glc produces a magenta precipitate after enzymatic hydrolysis. When the formulation design target is a warm-color signal channel, it can form a color difference with the blue-green signal of X-Glc, providing more options for multi-enzyme indicator formulation design and differentiated colony presentation.

✅ Diverse application directions, covering multiple types of microorganisms

The public application materials of Magenta-Glc involve Vibrio chromogenic culture media, Listeria chromogenic plates, and preliminary differentiation between Enterobacteriaceae and Salmonella. Multi-directional application references can expand product usage scenarios and are not limited to a single culture medium raw material selection.

✅ Domestic stock, more controllable procurement cost

Magenta-Glc is regularly stocked, with standard specifications of 1 g / 5 g / 25 g, and supports customized packaging. R&D teams can purchase in small batches according to the stage, reducing minimum order quantity pressure, shortening waiting cycles, and making overall procurement costs more controllable.