Chinese Name: Anaerobic Bacteria-β-D-Galactosidase Substrate

English Name: Anaerobic β-D-galactosidase substrate

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Lyophilized powder

Purity: HPLC ≥98%

Product Number: GST1011

Stock: In stock

Color: Red (insoluble precipitate)

The color development mechanism of the Anaerobic Bacteria-β-D-Galactosidase Red Substrate is consistent with that of the orange-yellow chromogenic substrate, both based on intramolecular condensation reaction – color development occurs without oxygen after enzymatic cleavage. The entire reaction process can be summarized in three stages: "cleavage – cyclization – color development":

1. Enzyme recognition and bond cleavage: β-Galactosidase specifically recognizes and hydrolyzes the β-D-galactoside bond in the substrate, releasing a colorless alditol dye precursor.

2. Intramolecular condensation: The released precursor molecule spontaneously undergoes intramolecular condensation without the need for oxygen or any auxiliary oxidant, forming a conjugated chromogenic structure.

3. Precipitation and color development: The condensation product is an insoluble red dye that precipitates in situ on the colony, helping to maintain in-situ colony color development.

Since the color development process does not rely on oxidative dimerization, stable and consistent color development results can be obtained under both aerobic and anaerobic culture conditions. Compared to traditional indole-based substrate systems that require oxygen for color development, this substrate is more suitable for applications such as anaerobic bacteria culture, screening, and identification.

✅ High purity control, ensuring detection consistency

• High purity quality control: HPLC ≥98% high-purity system ensures batch stability.

• Controllable background: Reduces non-specific chromogenic interference and minimizes background impact on interpretation.

✅ Anaerobic adaptation system, compatible with anaerobic detection

• Anaerobic color development: No oxygen required for reaction.

• Anaerobic/aerobic compatibility: Color development under both anaerobic and aerobic conditions.

• Application adaptability: Suitable for anaerobic bacteria culture and β-galactosidase screening systems.

✅ Red signal, high recognition

• Red precipitate signal: Forms red insoluble signal in situ after cleavage with clear signal boundaries.

• Clear contrast: Red color contrasts distinctly with the culture medium background, facilitating rapid identification of positive colonies.

• Intuitive interpretation: Reduces the risk of missing weakly positive samples and improves colony screening efficiency.

✅ Easy to operate, non-toxic intermediates

• No auxiliary reagents required: No need for oxidants or diazonium salts, simplifying the experimental process.

• Non-toxic intermediates: The color development process does not generate toxic intermediates, does not inhibit strain growth, and ensures detection sensitivity.

• Fewer experimental variables: Reduces operational errors and batch fluctuations introduced by auxiliary reagents, providing stable and reliable results.

✅ High quality at competitive price, in-stock supply

• In-stock supply: Sufficient inventory on hand, shortening procurement cycles and ensuring project progress.

• Price advantage: Leveraging the domestic supply chain, cost is significantly lower than imported products for the same quality.

• Custom services: Standard specifications of 1 g / 5 g / 25 g are available, with custom packaging supported to flexibly meet different project needs.